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mef2d antibody 5372  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mef2d antibody 5372
    Synergistic activation of IL-4 promoter by <t>ATF4-Mef2d-GATA3</t> axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.
    Mef2d Antibody 5372, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mef2d+antibody+5372/pmc12273732-47-23-26?v=Cell+Signaling+Technology+Inc
    Average 90 stars, based on 1 article reviews
    mef2d antibody 5372 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Oncostatin M Drives Th2 Polarized Allergic Airway Inflammation Through Fibroblast Reprogramming and Endoplasmic Reticulum Stress"

    Article Title: Oncostatin M Drives Th2 Polarized Allergic Airway Inflammation Through Fibroblast Reprogramming and Endoplasmic Reticulum Stress

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S535265

    Synergistic activation of IL-4 promoter by ATF4-Mef2d-GATA3 axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.
    Figure Legend Snippet: Synergistic activation of IL-4 promoter by ATF4-Mef2d-GATA3 axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.

    Techniques Used: Activation Assay, Immunoprecipitation, Confocal Microscopy, Transfection, Staining, Luciferase, Reporter Assay, Mutagenesis



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    mef2d antibody 5372  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc mef2d antibody 5372
    Synergistic activation of IL-4 promoter by <t>ATF4-Mef2d-GATA3</t> axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.
    Mef2d Antibody 5372, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mef2d+antibody+5372/pmc12273732-47-23-26?v=Cell+Signaling+Technology+Inc
    Average 90 stars, based on 1 article reviews
    mef2d antibody 5372 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Synergistic activation of IL-4 promoter by ATF4-Mef2d-GATA3 axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.

    Journal: International Journal of Nanomedicine

    Article Title: Oncostatin M Drives Th2 Polarized Allergic Airway Inflammation Through Fibroblast Reprogramming and Endoplasmic Reticulum Stress

    doi: 10.2147/IJN.S535265

    Figure Lengend Snippet: Synergistic activation of IL-4 promoter by ATF4-Mef2d-GATA3 axis.A, the amount of phosphorylated PERK (pPERK) in lung fibroblasts. ( B and C ) Immunoprecipitation showing ATF4-Mef2d interaction in lung fibroblasts from OSM-treated mice. ( D ) Predicted ATF4-Mef2d complex structure. ( E ) Confocal microscopy (630×) of subcellular His-Mef2d localization (in green) in ATF4-transfected HEK293 cells (the nuclei were stained with propidium iodide in red). ( F–H ) Cytoplasm Mef2d (F), nuclear Mef2d ( G ) and GATA3 ( H ) levels. E235 (5 µM; MCE): An inhibitor of ATF4. ( I ) Luciferase reporter assay of IL-4 promoter activation (A: ATF4; ( M ) Mef2d; ( G ) GATA3; muA: ATF4 mutant). ( J–K ) IL-4 mRNA ( I ) and protein ( J ) levels. GSK: (GSK2606414; 0.4 nM; MCE): An inhibitor of PERK. Data shown as mean ± SD with individual points. Statistical analysis: ANOVA with Tukey’s test; * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001. OD: Optical density.

    Article Snippet: Co-immunoprecipitation (Co-IP): Lung fibroblast lysates from OSM-treated mice were immunoprecipitated with ATF4 antibody (Santa Cruz, sc-390451) and Protein A/G beads, followed by WB analysis for Mef2d (Cell Signaling, 5372).

    Techniques: Activation Assay, Immunoprecipitation, Confocal Microscopy, Transfection, Staining, Luciferase, Reporter Assay, Mutagenesis